Proses fermentasi enzim yang dapat dimakan

Proses fermentasi tradisional

The production process of traditional fruit and vegetable enzymes generally has the following processes: raw material selection and impurity removal→cleaning→cutting→canning→sealed fermentation→filtration (fermentation liquid)→testing→bagging (sterilization), etc. Mainly put sugars, natural fruits and vegetables and water into ceramic fermentation tanks (without adding any other chemical substances) according to the golden ratio, and ferment at the mouth of the water-sealed tank. Open the tank for a certain period of time and stir, then seal and continue the fermentation at room temperature. Naturally ferment for six months to two years to obtain the plant essence, that is, the original enzyme prepared by the traditional enzyme fermentation process. Due to the simple operation of this process, it is often used as the main method of household enzyme production, of which the closed fermentation process is the most critical, which will affect the final product quality. Studies have shown that adding higher concentrations of isomaltose, sucrose or rice vinegar and other ingredients can effectively inhibit the growth of bacteria and the production of alcohol. However, traditional enzymes use natural fermentation technology, which is a closed fermentation system with mixed bacteria symbiosis, which has problems such as complicated parameter changes, difficult to control, and long fermentation cycle, which is not suitable for standardized production. In order to realize the large-scale industrialization and process production of edible enzymes, in recent years, researchers have mainly conducted research from two aspects: liquid fermentation and solid-state fermentation.

Fermentasi cair Produksi enzim pada tahap ini terutama mengadopsi fermentasi cair (juga disebut fermentasi terendam), di mana biji-bijian, buah-buahan, sayuran, jamur, tanaman obat dan dapat dimakan dan bahan baku lainnya diinokulasi dengan bakteri menguntungkan untuk fermentasi untuk meningkatkan bahan baku. Rasa, menghasilkan bahan aktif baru. Namun, produk fermentasi dari fermentasi cair pasti akan dipengaruhi oleh faktor-faktor seperti faktor musim, lingkungan fermentasi, dan mikroorganisme dalam sistem fermentasi. Oleh karena itu, setelah fermentasi, pembekuan vakum-pengeringan, pengeringan semprot dan cara teknis lainnya untuk menghilangkan sebagian besar air dalam enzim cair tidak hanya dapat memperoleh enzim padat yang masih memiliki aktivitas biologis, tetapi juga menghindari inaktivasi mikroorganisme selama transportasi dan penyimpanan. Fenomena seperti produksi gas-botol penggorengan, misalnya beras merah digunakan sebagai bahan baku untuk inokulasi probiotik dalam fermentasi cair dan dibekukan-kering dan dihancurkan untuk mendapatkan tepung enzim beras merah.

Solid-state fermentation The solid-state fermentation of edible enzymes is anhydrous fermentation on a solid substrate with a certain humidity. The solid fermentation substrate can be used as a fermentation substrate to provide carbon and nitrogen sources for the growth and reproduction of beneficial microorganisms, thereby promoting nutrient absorption and transformation. Research and practice have shown that filamentous fungi, bacteria and yeasts can ferment on solid substrates such as fruit peels and soybeans to produce enzymes, organic acids and other biologically active substances. For solid-state fermented enzymes, the types, content, and stability of the enzyme products are different due to their different fermentation conditions and conditions. However, from the current research, most of the raw materials for the production of enzymes contain a large amount of water and are not easy to carry out solid-state fermentation. The main problems in today's solid-state fermentation process include difficulty or insufficient mixing, and difficulty in controlling the fermentation rate and temperature during the fermentation process. Therefore, the solid-state fermentation edible enzyme technology needs to be further improved to meet the needs of large-scale industrial production.